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Beyotime
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Cellomics Technology LLC
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Promega
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Beijing CWBio
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HiMedia Laboratories
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Hoechst 33342, also known as bisbenzimide h33342 or hoe 33342, is a non embedding bright blfluorescent dye. Dyes have weak fluorescence in solution, and their fluorescence becomes bright after binding with DNA at the minor
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Image Search Results
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Paeoniflorin Attenuates Dexamethasone-Induced Apoptosis of Osteoblast Cells and Promotes Bone Formation via Regulating AKT/mTOR/Autophagy Signaling Pathway
doi: 10.1155/2021/6623464
Figure Lengend Snippet: Effect of paeoniflorin on DEX-induced apoptosis in MC3T3-E1 cells. (a, b) The extent of apoptosis of MC3T3-E1 cells was detected by flow cytometry after Annexin V-FITC/PI double staining. The apoptotic rate was measured by flow cytometric analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX. (c) MC3T3-E1 cells were stained by Hoechst to show the extent of apoptosis after interventions of DEX and paeoniflorin. (d) The expressions of osteogenic protein-Runx2 (runt-related transcription factor2) and apoptotic proteins such as Bcl-2 (B cell leukemia 2) and Bax (BCL2-associated X protein) were measured by western blot. ∗ P < 0.05, ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX; ## P < 0.01 vs. DEX. (e) Protein levels of caspase-3 and cleaved caspase-3 were determined by western blot analysis, shown in quantitative analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX.
Article Snippet: For Hoechst staining, 10 μ L
Techniques: Flow Cytometry, Double Staining, Control, Staining, Western Blot